Specimen Collection & Preparation

PLAC Test For Lp-PLA₂ - ELISA Format

Sample Collection

Collect blood samples in a serum or plasma collection tube, including those with a gel separator.
Process samples using standard separation procedures.
- Centrifuge and separate samples within four hours of venipuncture.
Store processed samples refrigerated at 2 to 8° C.
Transfer processed sample to test lab within 24 hours.
- If transporting the sample, ship overnight on cold packs sufficient to maintain 2 to 8° C or frozen (on dry ice).

Precautions

Exposure of samples to room temperature should be minimized to less than 6 hours (including blood draw, processing and transport time). This does not include incubation on the ELISA plate.
Storage at -20°C is not recommended.

Accession and Testing at Lab Site

Samples must be stored refrigerated at 2 to 8ºC for a minimum of 24 hours after the sample is drawn before testing can occur.
Samples can be tested up to 7 days after the sample is drawn when stored at
2 to 8ºC.
For longer term storage, serum/plasma samples must be stored at or below -70ºC. Once thawed, the sample can be tested up to 7 days when stored at
2 to 8ºC. Samples may be frozen and thawed up to 6 times without affecting the
Lp-PLA₂ quantitation.

Refer to PLAC Test Reagent Kit package insert for full recommendations on sample collection and storage.